KAR for TNBC
- Pharmacological profiling data. We obtained the HTS pharmacological profiling data of 12 TNBC cell lines from a recently published paper . The 12 TNBC cell lines are: BT20, BT549, CAL148, HCC38, HCC70, HCC1143, HCC1187, HCC1806, Hs578T, MDA-MB231, MDA-MB468 and MFM223.
- Quantitative kinase inhibition data. We obtained comprehensive quantitative kinase inhibition data for 72 of the 180 screened kinase inhibitors from literature. Drug sensitivity data from these 72 drugs were used in our algorithm. Quantitative kinase binding data was dichotomized as inhibited or not inhibited for each compound based on a threshold of IC50/Kd < 1μM or percent of inhibition > 85%. '0' indicates a kinase is inhibited by the drug and '1' indicates a kinase is not inhibited.
- Microarray gene expression data. We obtained the TNBC microarray gene expression data from the Cancer Cell Line Encyclopedia (GSE36133). These cell lines were profiled using Affymetrix HG-U133 Plus 2.0 microarrays. Raw CEL files for these cell lines were normalized using Robust Multiarray Average (RMA) approach in Affymetrix Power Tools (APT).
Fink LS, Beatty A, Devarajan K, Peri S, Peterson JR: Pharmacological profiling of kinase dependency in cell lines across triple-negative breast cancer subtypes. Molecular Cancer Therapeutics 2015, 14:298-306.